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亚傅体育app:教师简介

高向东
来源:亚傅体育app 时间:2016-12-26 12:02:32 阅读量:

    


职称职务:教授、博导
学科专业:微生物学
研究方向:酵母出芽与菌丝形成的调控
实验室位置:生科院大楼3017-3018室
联系电话:15327283490

Email: xdgao@whu.edu.cn

 

学习经历
1987-1992  中国科学技术大学生物系,学士
1992-1995  中国科学技术大学生物系,硕士
1995-1998  复旦大学遗传学研究所,博士

主要工作经历与任职情况:
1998-1999  美国佛蒙特大学(University of Vermont)医学院,博士后
1999-2006  美国宾夕法尼亚大学(University of Pennsylvania)医学院,博士后/研究助理
2006-至今  亚傅体育app,教授
2015-     微生物学系系主任


主要研究领域及兴趣:
1.解脂耶氏酵母菌丝形成的调控

  一些种类的真菌在碱性pH、高温、氮源缺乏等条件下能够从椭圆形的酵母形态转换成为菌丝或假菌丝,被称为二型性转换(dimorphic transition)。二型性转换被认为是真菌应对环境压力的一种反应,它也是某些致病性真菌的毒力因子之一。解脂耶氏酵母Yarrowia lipolytica是一种在进化上与酿酒酵母和白色念珠菌亲缘关系较远而与丝状真菌更为接近的酵母种类,对人无致病性,它具有强大的降解烷烃、脂肪酸和油脂的能力,在发酵工业中具有良好的应用前景。解脂耶氏酵母在葡萄糖等碳源存在时、碱性pH、低氧等条件下能够从椭圆形的酵母形态转换为菌丝或假菌丝,我们的目标是通过遗传学方法分离与鉴定参与调控解脂耶氏酵母二型性转换的基因,探索其作用机制,以增进对真菌二型性转换调控机制的认识。

2. 酿酒酵母出芽的调控

  酵母是单细胞真核微生物,绝大多数种类通过出芽进行繁殖。出芽是一种极性生长过程,包括细胞骨架的组织、囊泡运输和细胞壁的重塑,多种分子参与调控芽体的极性生长。酿酒酵母Saccharomyces cerevisiae是研究真核生物多种细胞学过程的模式生物之一,我们的目标是通过遗传学、细胞学和生物化学方法揭示酿酒酵母Rho GTP酶及其相互作用分子调控极性生长的机制,以增进对酵母出芽调控机制的认识。


承担课程(本科生、研究生):
1. 遗传学,全英文教学,弘毅班专业必修课(本科生),48学时

2. 高级微生物学,研究生专业必修课,48学时

3. 微生物学研究方法,研究生专业必修课,32学时


主持课题项目:
1. 国家自然科学基金面上项目(32070072),解脂耶氏酵母新型转录因子Fts1和Fts2负调控二型性转换的机制研究,2021-2024

2. 国家自然科学基金面上项目(31870062),酿酒酵母极性生长调控分子Boi2的功能机制研究,2019-2022

3. 国家自然科学基金面上项目(31570076),Ras信号传递途径下游效应分子Mhy1与YlRim15在解脂耶氏酵母二型性转换中的功能与调控机制研究,2016-2019

4. 国家自然科学基金面上项目(31370124),酿酒酵母极性生长关键调控分子Rho3的新调控因子、下游效应分子及功能的鉴定,2014-2017

5. 国家自然科学基金面上项目(30871347),酿酒酵母 Rho GTP酶在细胞极性建立过程中调控机制的研究,2009-2011

6. 国家自然科学基金面上项目(30770017),酿酒酵母Bud3p对septin细胞骨架调节机制的研究,2008-2010


发表的论文(*通讯作者):
1. Shu T, He X-Y, Chen J-W, Mao Y-S, Gao X-D* (2021) The pH-responsive transcription factors YlRim101 and Mhy1 regulate alkaline pH-induced filamentation in the dimorphic yeast Yarrowia lipolytica. mSphere 6(3): e00179-21.

2. Zhu J, Jia Z-W, Xia C-Y, Gao X-D* (2020) The Sur7/PalI family transmembrane protein Tos7 (Yol019w) plays a role in secretion in budding yeast. Fungal Genet. Biol. 144: 103467.

3. Jia Z-W, Lv S-X, Zhu J, Xia C-Y, Gao X-D* (2020) Roles of the PH, coiled-coil and SAM domains of the yeast polarity protein Boi2 in polarity-site localization and function in polarized growth. Curr. Genet. 66(6): 1101-1115.

4. Wu H, Shu T, Mao Y-S, Gao X-D* (2020) Characterization of the promoter, downstream target genes and recognition DNA sequence of Mhy1, a key filamentation-promoting transcription factor in the dimorphic yeast Yarrowia lipolytica. Curr. Genet. 66(1): 245-261.

5. Nie W-C, He F, Yuan S-M, Jia Z-W, Wang R-R, Gao X-D* (2017) Roles of an N-terminal coiled-coil-containing domain in the localization and function of Bem3, a Rho GTPase-activating protein in budding yeast. Fungal Genet. Biol. 99: 40-51.

6. Liang S-H, Wu H, Wang R-R, Wang Q, Shu T, Gao X-D* (2017) The TORC1-Sch9-Rim15 signaling pathway represses yeast-to-hypha transition in response to glycerol availability in the oleaginous yeast Yarrowia lipolytica. Mol. Microbiol. 104(4):553-567.

7. Yuan S-M, Nie W-C, He F, Jia Z-W, Gao X-D* (2016) Kin2, the budding yeast ortholog of animal MARK/PAR-1 kinases, localizes to the sites of polarized growth and may regulate septin organization and the cell wall. PLoS ONE 11(4): e0153992.

8. Wu H, Guo J, Zhou Y-T, Gao X-D* (2015) The anillin-related region of Bud4 is the major functional determinant for Bud4’s function in septin organization during bud growth and axial bud-site selection in budding yeast. Eukaryot. Cell 14(3): 241-251.

9. He F, Nie W-C, Tong Z, Yuan S-M, Gong T, Liao Y, Bi E, Gao X-D* (2015) The GTPase-activating protein Rga1 interacts with Rho3 GTPase and may regulate its function in polarized growth in budding yeast. PLoS ONE 10(4): e0123326.

10. Li Y-Q, Li M, Zhao X-F, Gao X-D* (2014) A role for the Rap GTPase YlRsr1 in cellular morphogenesis and the involvement of YlRsr1 and the Ras GTPase YlRas2 in bud-site selection in the dimorphic yeast Yarrowia lipolytica. Eukaryot. Cell 13(5): 580-590.

11. Li M, Li Y-Q, Zhao X-F, Gao X-D* (2014) Roles of the three Ras proteins in the regulation of dimorphic transition in the yeast Yarrowia lipolytica. FEMS Yeast Res. 14(3): 451-463.

12. Gong T, Liao Y, He F, Yang Y, Yang D-D, Chen X-D, Gao X-D* (2013) Control of polarized growth by the Rho family GTPase Rho4 in budding yeast: Requirement of the N-terminal extension of Rho4 and regulation by the Rho GTPase-activating protein Bem2. Eukaryot. Cell 12(2): 368-377.

13. Liao Y, He F, Gong T, Bi E, Gao X-D* (2013) Msb1 interacts with Cdc42, Boi1, and Boi2 and may coordinate Cdc42 and Rho1 functions during early stage of bud development in budding yeast. PLoS One 8:e66321.

14. Zhao XF, Li M, Li YQ, Chen XD, Gao X-D* (2013) The TEA/ATTS transcription factor YlTec1p represses the yeast-to-hypha transition in the dimorphic yeast Yarrowia lipolytica. FEMS Yeast Res. 13:50-61.

15. Tang Y, Gao X-D, Wang Y, Yuan BF, Feng YQ (2012) Widespread existence of cytosine methylation in yeast DNA measured by gas chromatography/mass spectrometry. Anal. Chem. 84:7249-55.

16. Guo J, Gong T, Gao X-D* (2011) Identification of an amphipathic helix important for the formation of ectopic septin spirals and axial budding in yeast axial landmark protein Bud3p. PLoS One 6:e16744.

17. Tong Z, Gao X-D, Howell AS, Bose I, Lew DJ, Bi E (2007) Adjacent positioning of cellular structures enabled by a Cdc42 GTPase-activating protein-mediated zone of inhibition. J. Cell Biol. 179:1375-1384.

18. Gao X-D, Sperber LM, Kane SA, Tong Z, Tong AHY, Boone C, Bi E (2007) Sequential and distinct roles of the cadherin domain-containing protein Axl2p in cell polarization in yeast cell cycle. Mol. Biol. Cell 18:2542-2560.

19. Tcheperegine SE, Gao X-D, Bi E (2005) Regulation of cell polarity by interactions of Msb3 and Msb4 with Cdc42 and polarisome components. Mol. Cell. Biol. 25:8567-8580.

20. Gao X-D, Caviston JP, Tcheperegine SE, Bi E (2004) Pxl1p, a paxillin-like protein in Saccharomyces cerevisiae, may coordinate Cdc42p and Rho1p functions during polarized growth. Mol. Biol. Cell 15:3977-3985.

21. Grimme SJ, Gao X-D, Martin PS, Tu K, Tcheperegine SE, Corrado K, Farewell AE, Orlean P, Bi E (2004) Deficiencies in the endoplasmic reticulum (ER)-membrane protein Gab1p perturb transfer of glycosylphosphatidylinositol to proteins and cause perinuclear ER-associated actin bar formation. Mol. Biol. Cell 15:2758-2770.


22. Gao X-D, Albert S, Tcheperegine SE, Burd CG, Gallwitz D, Bi E (2003) The GAP activity of Msb3p and Msb4p for the Rab GTPase Sec4p is required for efficient exocytosis and actin organization. J. Cell Biol. 162:635-646.



             

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